Alexa Fluor(R) 647 anti-human SSEA-4 330407 from BioLegend

Product Specs
ItemAlexa Fluor(R) 647 anti-human SSEA-4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number330407
IsotypeIgG3, Kappa
Quantity25 tests
CloneMC-813-70
Conjugate/TagAlexa Fluor® 647
HostMouse
ReactivityHuman
ApplicationsFlow Cytometry (FC)
Antibody TypePrimary
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer
ImmunogenHuman embryonic carcinoma cell line 2102Ep
ClonalityMonoclonal
TargetSSEA-4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Generation of CHOPe003-A ESC line to study an ACTG2 variant affecting smooth muscle development and functionStem cell researchSeptember 1, 2023Hashmi SK, Schneider S, Gagne AL, Maguire JA, Anderson S, Gadue P, Heuckeroth RO, French DL.anti-Sox2 (D6D9),1:50,Biolegend #330306,RRID:AB_1279440 ,AF488 anti-human SSEA-3,1:400,Biolegend #330408,RRID:AB_1089200 ,AF647 anti-human SSEA-4,1:100,Biolegend #330614,RRID:AB_2119064 ,AF488 anti-human

(2) Generation of a human Tropomyosin 1 knockout iPSC lineStem Cell ResearchSeptember 1, 2023Madison B. Wilken, Jean Ann Maguire, Lea V. Dungan, Alyssa Gagne, Catherine Osorio-Quintero, Elisa A Waxman, Stella T. Chou, Paul Gadue, Deborah L. French, Christopher S. ThomCat# 330306, RRID: AB_1279440" Pluripotency Markers (Flow Cytometry),SSEA-4,1:400,"BioLegend, Cat# 330408, RRID: AB_1089200" Pluripotency Markers (Flow Cytometry),SSEA-5,1:200,"BioLegend, Cat# 355210, RRID:

(3) Generation of 2 isogenic clones from a patient with Trisomy 21 and a GATA1 mutationStem Cell ResearchJune 1, 2023Kaoru Takasaki, Sara S. Kumar, Alyssa Gagne, Deborah L. French, Stella T. ChouSSEA-3,1:50,BioLegend #330306,RRID:AB_1279440 Pluripotency Markers,AF647 anti-human SSEA-4,1:400,BioLegend #330408,RRID:AB_1089200 Pluripotency Markers,AF488 anti-human Tra-1-60,1:100,BioLegend #330614,RRID:AB_2119064 Pluripotency

(4) Generation of a human Tropomyosin 1 knockout iPSC linebioRxiv : the preprint server for biologyMay 4, 2023Wilken MB, Maguire JA, Dungan LV, Gagne A, Osorio-Quintero C, Waxman EA, Chou ST, Gadue P, French DL, Thom CS.AB_493257" ,SSEA-3,1:50,"BioLegend, Cat# 330306, RRID: AB_1279440" ,SSEA-4,1:400,"BioLegend, Cat# 330408, RRID: AB_1089200" ,SSEA-5,1:200,"BioLegend, Cat# 355210, RRID: AB_2562013" ,Tra-1–60,1:50,"BioLegend,

(5) An induced pluripotent stem cell line (CIMRi001-A) from a Vici syndrome donor with a homozygous recessive c.1007A>G (p.Q336R) mutation in the EPG5 geneStem Cell ResearchAugust 1, 2022Matthew W. Mitchell, Christine Grandizio, Nahid Turan, Deborah V. Requesensfor immunocytochemistry/flow-cytometry ,Antibody,Dilution,Company Cat # and RRID ,,, Pluripotency Markers,Alexa Fluor 647 anti human SSEA4,1:200,"BioLegend Cat# 330408, RRID:AB_1089200" Pluripotency Markers,Alexa Fluor 647 Mouse IgG3 κ,1:300,"BioLegend Cat# 401321, RRID:

(6) Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signaturesNature communicationsJune 7, 2022Bi Y, Tu Z, Zhou J, Zhu X, Wang H, Gao S, Wang Y.following primary antibodies used in this study were used: APC-conjugated anti human SSEA4 (BioLegend, Cat#330407,RRID: AB_1089201—1:50 dilution), APC-conjugated anti-KRT7 (Abcam, Cat#ab192077, Lot GR3214132-7,1:500

(7) Multi-omic rejuvenation of human cells by maturation phase transient reprogrammingeLifeApril 8, 2022Gill D, Parry A, Santos F, Okkenhaug H, Todd CD, Hernando-Herraez I, Stubbs TM, Milagre I, Reik W.dissociation reagent and incubated with antibodies against CD13 (PE, 301704, BioLegend), SSEA4 (AF647, 330408, BioLegend), and CD90.2 (APC-Cy7, 105328, BioLegend) for 30 min. Cells were washed two times with 2%

(8) Generation of an induced pluripotent stem cell (iPSC) line (JUCTCi017-A) from a patient with limb-girdle muscular dystrophy (LGMD) due to a homozygous p.Lue287Ser fs14* mutation in the SGCB geneStem cell researchJuly 1, 2021Ababneh NA, Barham R, Al-Kurdi B, Ali D, Sharar N, Al Hadidi S, Alatoom RM, Zalloum S, Gharandouq MH, Makahleh L, et al.

(9) Generating an iPSC line (with isogenic control) from the PBMCs of an ACTA1 (p.Gly148Asp) nemaline myopathy patientStem cell researchJuly 1, 2021Houweling PJ, Coles CA, Tiong CF, Nielsen B, Graham A, McDonald P, Suter A, Piers AT, Forbes R, Ryan MM, et al.

(10) Antibody-mediated depletion of CCR10+EphA3+ cells ameliorates fibrosis in IPFJCI insightJune 8, 2021Hohmann MS, Habiel DM, Espindola MS, Huang G, Jones I, Narayanan R, Coelho AL, Oldham JM, Noth I, Ma SF, et al.streptavidin (Thermo Fisher Scientific: SA1001), Alexa Fluor 647–conjugated anti-SSEA4 (BioLegend: 330408) or biotinylated anti-SSEA4 (BioLegend: 330404) plus FITC-conjugated streptavidin (Thermo Fisher Scientific:

(11) Generation of a double insulin and somatostatin reporter line, SCSe001-A-3, for the advancement of stem cell-derived pancreatic isletsStem cell researchDecember 8, 2020Leavens KF, Liao CM, Gagne AL, Kishore S, Cardenas-Diaz FL, French DL, Gadue P.Cat# 330306, RRID: AB_1279440" Pluripotency Markers (Flow cytometry),SSEA-4,1:400,"Biolegend, Cat# 330408, RRID: AB_1089200" Pluripotency Markers (Flow cytometry),Tra-1-60,1:50,"Biolegend, Cat# 330614, RRID:

(12) Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton's jelly samplesStem cell researchDecember 1, 2020Ababneh NA, Al-Kurdi B, Barham R, Ali D, Sharar N, Abuarqoub D, Jafar H, Salah B, Awidi A.

(13) Generation and characterization of induced pluripotent stem cell (iPSC) line (JUCTCi002-A) from a patient with ataxia with oculomotor apraxia type 1 (AOA1) harboring a homozygous mutation in the APTX geneStem cell researchOctober 1, 2020Ababneh NA, Al-Kurdi B, Ali D, Abuarqoub D, Barham R, Alzibdeh AM, Khanfar AN, Altantawi AM, Ryalat AT, Sharrack B, et al.

(14) Establishment of a human induced pluripotent stem cell line, JUCTCi012-A, from multiple symmetric lipomatosis (MSL) patient carrying a homozygous Arg707Trp (c.2119C > T) mutation in the MFN2 geneStem cell researchOctober 1, 2020Ababneh NA, Ali D, Barham R, Al-Kurdi B, Sharar N, Al Hadidi S, Qanno' O, Ryalat AT, Salah B, Awidi A.

(15) Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC linesStem cell researchMay 1, 2020Döpper H, Horstmann M, Menges J, Bozet M, Kanber D, Steenpass L.

(16) Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency RegulationCell reportsMarch 17, 2020Bi Y, Tu Z, Zhang Y, Yang P, Guo M, Zhu X, Zhao C, Zhou J, Wang H, Wang Y, et al.

(17) Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editingStem cell researchJanuary 1, 2020Nur Patria Y, Lilianty J, Elefanty AG, Stanley EG, Labonne T, Bateman JF, Lamandé SR.

(18) Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous stateStem cell researchAugust 1, 2019Menges J, Cremanns M, Steenpass L.

(19) Generation of a human iPSC line from a patient with Marfan syndrome caused by mutation in FBN1Stem cell researchApril 1, 2019Li X, Dong T, Li Y, Wu F, Lan F.

(20) Transient activation of the UPR^ER is an essential step in the acquisition of pluripotency during reprogrammingScience advancesApril 1, 2019Simic MS, Moehle EA, Schinzel RT, Lorbeer FK, Halloran JJ, Heydari K, Sanchez M, Jullié D, Hockemeyer D, Dillin A.dissociated with TrypLE and pelleted. A 100-μl fluorescent-conjugated antibodies cocktail (5 μl of SSEA-4 330408 and 5 μl of TRA-1-60 330610; BioLegend) in staining media (1× PBS and 2% FBS) was used to resuspend

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Alexa Fluor(R) 647 anti-human SSEA-4 from BioLegend

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Alexa Fluor(R) 647 anti-human SSEA-4 from BioLegend

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